Academic Perseverance: the time I quit my PhD

By Ruenda Loots

Although many people want to know how long it takes to finish a PhD, the more important question is “What does it take to finish a PhD?” Perhaps the most significant characteristic of successful post-graduate researchers is grit. Sticking to it when everything comes undone. And sometimes, picking up the pieces and starting again.

When the going got tough
When the going got tough

Two years into my PhD and the only word that accurately described my research to date was “abysmal”. As I’ve mentioned before, I’m not a microbiologist. I knew the project would be mostly microbiology but it sounded like an exciting challenge! The novelty wore off very quickly and what looked exciting 12 months before became an insurmountable mountain of work.

The theory eluded me. I could barely frame research questions because my fundamental knowledge was lacking. I would read the introduction of an article and feel so overwhelmed by everything I didn’t know that I obsessively downloaded every reference in the article, which in turn led me to download even more articles until I had folders in folders of unread articles that were labelled NB, Must read and Very Important.

The practical work intimidated me. Never before was I concerned about a sterile workbench. I was so paranoid I used to mark a 20cm ring around my gas flame with masking tape so that I wouldn’t accidentally move outside The Clean Zone (but since I had two left-hands it didn’t help much). I melted a couple of latex gloves onto my fingers that year.

Days became months with no progress, months became semesters and each passing calendar page made me realise: I can’t do this. I prepared to give up.

Leaving my (dis)comfort zone

Toronto skyline (Pixabay)
Toronto skyline (Pixabay)

Then, at the end of Year Two, one of my supervisor’s collaborators invited me to visit his laboratory at Ryerson University. I would work closely with a post-doc to learn advanced microscopic techniques which were vital for my research. I was just married and had no desire to leave my new happiness behind but I had no alternatives for my ongoing academic despair so I boarded a plane to Toronto, Canada, for the two-week visit.

Reflecting on the experience three years later reveals how valuable it really was. I established great relationships (scientific and social). The post-doc (now a close friend) taught me with great patience how to use a fancy microscope and more importantly how to do the very basics I had struggled with for so long. There are things that books and articles can’t teach you – an encouraging, open-minded mentor is the only way.

Zen and the art of biofilm analysis

Toronto Harbour (Pixabay)
Toronto Harbour (Pixabay)

Every evening I strolled through the unfamiliar city. One night I found “The World’s Biggest Book Store” and bought a book that changed my scientific career in the most unexpected ways. Zen and the Art of Motorcycle Maintenance crossed my path at exactly the right moment. This book’s explanation of the philosophy of science, using a motorcycle as metaphor, stirred a passion in me that had been lost in the years of “I can’t do this.”

The solutions all are simple… after you have arrived at them. But they’re simple only when you know already what they are”

On my last night in Toronto I wrote a letter to myself titled: You almost quit your PhD. I wrote down all the things that scared me about the PhD. I wrote down all the things I couldn’t do. I wrote down all the fears of failure. I returned to Cape Town and handed the sealed envelope to my husband.

“Give this to me on the day I graduate, okay?”

I will read it at the end of this year.

Poo-rification

By Keafon Jumbam

I recently attended three weeks of intensive laboratory training on fecal hormone assays – okay, let’s be honest: poop analysis. If you’re thinking- eeuw, you are not alone. Before embarking on this PhD degree, collecting poop didn’t cross my mind. I mean, who thinks about poop, right? Except, hard-core dieters and maybe crazy scientists? And now I’m one of them, patiently waiting on batties to – em – crap so I can scoop it up, much to the surprise of the batties themselves. But guess what, there’s plenty of power in poo. Let me explain.

Lab work involves eating, naturally.
Lab work involves eating, naturally.

When stressed, an animal releases stress hormones into its system, which is commonly measured through blood extraction. But therein lies the catch: the very act of capturing and drawing blood from an animal is not only cumbersome but could contribute to further stress. And that’s not all; licensed personnel and strict ethical requirements are needed to carry out such operations. Thanks to recent advances in science, you can avoid all the above logistics by simply collecting poop. All you need do is wait for the animal to do its business – which in my case is pretty easy, since we spend time with them daily collecting behavioural data. The best part is that it’s a non-invasive and simple method of collecting scientific data without interrupting the animal’s normal activities. Once collected, it is crucial to freeze the samples immediately to prevent hormonal degradation.

Poop pulverization
Poop pulverization

The fun begins in the lab when we play around with chemical solutions and extract hormones from the samples. Firstly, you freeze-dry the samples and then crush them before extracting the hormones. So now you have your hormone extract – yippee, but how much of it is in your sample? Caution: plenty of calculations and pipetting involved!

Practice makes perfect
Practice makes perfect

A colleague and I had some pretty embarrassing moments as we quickly reached out for our cell phones when asked to do simple calculations and unit conversions. “You may be PhD students out there but in here, you are elementary level” a staff member joked. It didn’t help that our pipetting skills got worse as the weeks progressed – the harder we focused, the worse we got. “When you focus too hard, you tense up and make more errors. You need to relax and get into a rhythm that works for you” was the advice we got from the experts. We’re tough field biologists – who knew transferring liquids in a controlled environment could be so tricky?!

Stephanie, our patient poop tutor
Stephanie, our patient poop tutor

Despite our failures, the exposure and experience we gained were invaluable. It was also comforting to know that it took the professionals several months of trial and error to master their art; we couldn’t possibly get it right in just three weeks. I must admit I have new respect for the challenges of a lab environment – it’s not all as straightforward as I’d thought. I went home in high spirits, looking forward to my next lab visit to perfect my skills. After all, practice makes perfect, or in this case, poo-fect!